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Footnotes Edited by: Hartmut Jaeschke, University of Kansas Medical Center Research Institute, United States Reviewed by: Anup Ramachandran, University of Kansas Medical Center, United States Noé López-Amador, Universidad Veracruzana, Mexico Data availability statement The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors
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In prior studies, we found that the reversible covalent inhibitor GC376 would not shift the mass of 3CL in MALDI MS due to the sensitivity of its covalent bond to pH changes, but pre-incubation of the 3CL protease with one equivalent of GC376 would occupy the active site and block the subsequent binding of Compound 4 to 3CL protease
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