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The following time program was used with a flow rate of 0.3 mL per minute: A solution of 4-hydroxynaphthalene-1-carbaldehyde (1) (10.0 g, 58.1 mmol) in dry THF (200 mL) was cooled to 0° C., and imidazole (12.0 g, 174 mmol) and tert-butyldimethylsilyl chloride (TBSCl) (13.1 g, 87.1 mmol) were added sequentially

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Therefore, we hypothesized that GCLC downregulation under high glucose conditions was a function of reduced HuR subcellular localization to the cytoplasm, as previously observed in cell culture models under these conditions ( 17, 26 )