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Briefly, reagents were added to a 0.5 mL microfuge tube in the following order: 0.5 μg of plasmid DNA, 20 mM Tris-HCl steril pH 7.4 buffer, 10.8 mg/mL of GSGa, 100 μM of either H 2 O 2 or CuCl 2 or CoCl 2 and 10 mM ascorbic acid, and sterilized ddH 2 O to a final volume of 10 μL
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